Journal: Toxins

Article Title: Co-Expression and Co-Purification Enable Manufacturing of a Six-Monoclonal Antibody Botulinum Antitoxin Cocktail

doi: 10.3390/toxins18050199

Figure Lengend Snippet: IEX HPLC Analysis of 5 mL HiTrap Capto S Column Fraction 15 (blue) and S column load (black). The inset shows the protein concentration measured by A280 on the Nanodrop spectrophotometer for the collected peak fractions (1 mL per fraction). Fraction 15 in the histogram is highlighted in blue. (IEX-HPLC of individual purified antibodies is shown in .) The column was loaded with 20 μg antibody.

Article Snippet: Cytiva HiTrap Capto MMC, Cytiva MabSelect PrismA, Cytiva Fibro PrismA, Eshmuno CP-FT, Cytiva Capto Q (1 mL), Cytiva Capto S (1 mL), Cytiva Capto Adhere (1 mL), MabSelect SuRe resin, MilliporeSigma ViResolve Modus 1.1 viral reduction filter (Sigma, St. Louis, MO, USA), Pall (Port Washington, NY, USA) Cadence Omega 0.1 m 2 30 kDa TFF cassette.

Techniques: Protein Concentration, Spectrophotometry, Purification

Journal: Microorganisms

Article Title: Molecular Characterization of Novel Family IV and VIII Esterases from a Compost Metagenomic Library

doi: 10.3390/microorganisms9081614

Figure Lengend Snippet: Gels of purified Est8L ( a ) and Est13L ( b ) after SDS-PAGE and staining with Coomassie blue. Red arrows in ( a ) and ( b ) indicate the predicted positions of Est8L and Est13L, respectively. C, crude extract; Q, HiTrap Q fraction; M, HiTrap capto MMC fraction; H, t -butyl HIC fraction.

Article Snippet: Columns for purification such as HiTrap Q HP (5 mL), t -butyl hydrophobic interaction chromatography (HIC) (1 mL), HiTrap capto MMC (1 mL), and HiPrep 16/60 Sephacryl S-200 HR were purchased from GE Healthcare (Uppsala, Sweden).

Techniques: Purification, SDS Page, Staining

Journal: Microorganisms

Article Title: Molecular Characterization of Novel Family IV and VIII Esterases from a Compost Metagenomic Library

doi: 10.3390/microorganisms9081614

Figure Lengend Snippet: Purification of Est8L and Est13L using chromatographic procedures.

Article Snippet: Columns for purification such as HiTrap Q HP (5 mL), t -butyl hydrophobic interaction chromatography (HIC) (1 mL), HiTrap capto MMC (1 mL), and HiPrep 16/60 Sephacryl S-200 HR were purchased from GE Healthcare (Uppsala, Sweden).

Techniques: Purification, Activity Assay

Journal: Microorganisms

Article Title: Molecular Characterization of Novel Family IV and VIII Esterases from a Compost Metagenomic Library

doi: 10.3390/microorganisms9081614

Figure Lengend Snippet: Activity staining of Est8L ( a ) and Est13L ( b ) onto the agar strips after native-PAGE and analysis of recovered Est8L ( c ) and Est13L ( d ) eluted from the activity-stained agar strips using SDS-PAGE and staining with silver nitrate. Native-PAGE was performed using the HiTrap Q fraction with the highest activity, and activity staining was performed according to the procedure described in the Materials and Methods. Red arrows in ( a , b ) represent the active bands for the enzymes. Red arrows in ( c , d ) indicate the predicted positions of Est8L and Est13L, respectively. S, size markers; C, crude extracts; Q, fraction pools from HiTrap Q column chromatography; A, pools from activity staining.

Article Snippet: Columns for purification such as HiTrap Q HP (5 mL), t -butyl hydrophobic interaction chromatography (HIC) (1 mL), HiTrap capto MMC (1 mL), and HiPrep 16/60 Sephacryl S-200 HR were purchased from GE Healthcare (Uppsala, Sweden).

Techniques: Activity Assay, Staining, Clear Native PAGE, SDS Page, Column Chromatography